The value of neuroendocrine markers in non-small cell lung cancer: a comparative immunohistopathologic study
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kiriakogiani-Psaropoulou, P.
Malamou-Mitsi, V.
Martinopoulou, U.
Legaki, S.
Tamvakis, N.
Vrettou, E.
Fountzilas, G.
Skarlos, D.
Kosmidis, P.
Pavlidis, N.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Lung Cancer
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
In order to estimate the value of immunohistochemical identification of neuroendocrine (NE) differentiation markers in non-small cell lung carcinomas (NSCLCs), we investigated the expression of five neuroendocrine and neural differentiation-related antigens in 51 NSCLCs. Additionally, 20 epithelial lung tumors with NE differentiation [15 carcinoids and five small cell lung carcinomas (SCLCs)] and 61 epithelial tumors of various other origin (breast, prostate, colon and head-neck carcinomas) were studied. An indirect two-stage immunoperoxidase method was performed in formalin-fixed and paraffin-embedded tissue specimens, by using commercially available monoclonal antibodies. These antibodies are directed against neuron-specific enolase (NSE), chromogranin-A and Leu-7 which are general markers of NE differentiation, bombesin, which is a specific NE secretory product and neurofilament triplet protein (NFTP), an intermediate filament protein of neuronal differentiation. All five markers demonstrated a positive immunoreactivity in NSCLCs, equally distributed to all three histologic subtypes, ranging from 16 to 47% of the cases (NSE 47%, bombesin 21.5%, Leu-7 21.5%, chromogranin-A 18% and NFTP 16%). Most of the carcinoids and SCLCs expressed multiple or all NE markers. The other four epithelial tumors showed a positive immunoreactivity for bombesin, Leu-7 and NFTP, ranging from 11 to 40% of the cases. Chromogranin-A was not expressed in any of these tumors, whereas NSE was demonstrated only in 17% of breast carcinomas. The following remarks can be drawn from this study: (1) some NSCLCs showed immunophenotypic NE differentiation; (2) among all the markers used, NSE was the most sensitive (sensitivity, 100%) and chromogranin-A the most specific (specificity, 100%); and (3) NSE and chromogranin-A appear to be the most valuable and useful indicators of probable neuroendocrine differentiation in lung epithelial tumors.
Περιγραφή
Λέξεις-κλειδιά
Antigens, CD/analysis, Antigens, CD57, Antigens, Differentiation, T-Lymphocyte/analysis, Bombesin/analysis, Carcinoma, Non-Small-Cell Lung/*pathology, Chromogranin A, Chromogranins/analysis, Humans, Immunoenzyme Techniques, Lung Neoplasms/*pathology, Neurofilament Proteins/analysis, Phosphopyruvate Hydratase/analysis, Tumor Markers, Biological/*analysis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/7535640
http://ac.els-cdn.com/0169500294921644/1-s2.0-0169500294921644-main.pdf?_tid=acc56114eda3d09cfd9a18774d4a09b6&acdnat=1333611903_c4a720d229fc9572b02e4a65a2f995bb
http://ac.els-cdn.com/0169500294921644/1-s2.0-0169500294921644-main.pdf?_tid=acc56114eda3d09cfd9a18774d4a09b6&acdnat=1333611903_c4a720d229fc9572b02e4a65a2f995bb
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής