The position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope
Φόρτωση...
Ημερομηνία
Συγγραφείς
Alexopoulos, C.
Tsikaris, V.
Rizou, C.
Sakarellos-Daitsiotis, M.
Sakarellos, C.
Cung, M. T.
Marraud, M.
Vlachoyiannopoulos, P. G.
Moutsopoulos, H. M.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Wiley
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Biopolymers
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
A sequential oligopeptide carrier of antigenic peptides is presented, incorporating two Aib residues in each repetitive moiety: Ac-(Aib-Lys-Aib-Gly)(n), (SOCn-II; n = 2-4). The conformational study, by H-1-nmr, CD, and Fourier transform ir spectroscopy; indicated that the SOCn-II carrier displays a pronounced 3(10)-helix, compared to the Ac-(Lys-Aib-Gly)(n) (SOCn-I) carrier of the same approximately backbone length, previously reported One of the dominant autoimmune epitopes of the Sm and U1RNP cellular components, the PPGMRPP sequence, was coupled to the Lys-(NH2)-H-epsilon groups of the SOCn-II carrier and used as antigenic substrate for detecting anti-Sm/ U1RNP autoantibodies in ELISA assays. Anti-Sm antibodies are highly specific for systemic lupus erythematosus, while anti-U1RNP are specific for mixed connective tissue disease. The anti-(PPGMRPP)(5)-SOCn-II ELISA was compared with the anti-(PPGMRPP)(n)-SOCn-I ELISA, provided that both antigenic substrates possess the same amount of the epitope replicates. The significance of the lysine positions along the oligopeptide backbone of the carrier for a favorable antibody recognition of the anchored antigens is also examined. (C) 2000 John Wiley & Sons, Inc. Biopoly 54: 1-10, 2000.
Περιγραφή
Λέξεις-κλειδιά
sequential oligopeptide carrier, antigenic peptides, socn, sm antigen, 3(10)-helix, circular-dichroism spectra, synthetic-peptide, repetitive epitope, alpha-helices, amino-acids, spectroscopy, specificity, secondary, protein, design
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000087289500001
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1097-0282(200007)54:1<1::AID-BIP10>3.0.CO;2-7/asset/10_ftp.pdf?v=1&t=h0e0ip2g&s=5cb0295ab7c8a3bd303ef0df6736f300becfb4e2
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1097-0282(200007)54:1<1::AID-BIP10>3.0.CO;2-7/asset/10_ftp.pdf?v=1&t=h0e0ip2g&s=5cb0295ab7c8a3bd303ef0df6736f300becfb4e2
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας