The position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope

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dc.contributor.authorAlexopoulos, C.en
dc.contributor.authorTsikaris, V.en
dc.contributor.authorRizou, C.en
dc.contributor.authorSakarellos-Daitsiotis, M.en
dc.contributor.authorSakarellos, C.en
dc.contributor.authorCung, M. T.en
dc.contributor.authorMarraud, M.en
dc.contributor.authorVlachoyiannopoulos, P. G.en
dc.contributor.authorMoutsopoulos, H. M.en
dc.date.accessioned2015-11-24T16:50:59Z
dc.date.available2015-11-24T16:50:59Z
dc.identifier.issn0006-3525-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/9669
dc.rightsDefault Licence-
dc.subjectsequential oligopeptide carrieren
dc.subjectantigenic peptidesen
dc.subjectsocnen
dc.subjectsm antigenen
dc.subject3(10)-helixen
dc.subjectcircular-dichroism spectraen
dc.subjectsynthetic-peptideen
dc.subjectrepetitive epitopeen
dc.subjectalpha-helicesen
dc.subjectamino-acidsen
dc.subjectspectroscopyen
dc.subjectspecificityen
dc.subjectsecondaryen
dc.subjectproteinen
dc.subjectdesignen
dc.titleThe position of the LysN(epsilon)H(2)-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)(n) (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitopeen
heal.abstractA sequential oligopeptide carrier of antigenic peptides is presented, incorporating two Aib residues in each repetitive moiety: Ac-(Aib-Lys-Aib-Gly)(n), (SOCn-II; n = 2-4). The conformational study, by H-1-nmr, CD, and Fourier transform ir spectroscopy; indicated that the SOCn-II carrier displays a pronounced 3(10)-helix, compared to the Ac-(Lys-Aib-Gly)(n) (SOCn-I) carrier of the same approximately backbone length, previously reported One of the dominant autoimmune epitopes of the Sm and U1RNP cellular components, the PPGMRPP sequence, was coupled to the Lys-(NH2)-H-epsilon groups of the SOCn-II carrier and used as antigenic substrate for detecting anti-Sm/ U1RNP autoantibodies in ELISA assays. Anti-Sm antibodies are highly specific for systemic lupus erythematosus, while anti-U1RNP are specific for mixed connective tissue disease. The anti-(PPGMRPP)(5)-SOCn-II ELISA was compared with the anti-(PPGMRPP)(n)-SOCn-I ELISA, provided that both antigenic substrates possess the same amount of the epitope replicates. The significance of the lysine positions along the oligopeptide backbone of the carrier for a favorable antibody recognition of the anchored antigens is also examined. (C) 2000 John Wiley & Sons, Inc. Biopoly 54: 1-10, 2000.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondary<Go to ISI>://000087289500001-
heal.identifier.secondaryhttp://onlinelibrary.wiley.com/store/10.1002/(SICI)1097-0282(200007)54:1<1::AID-BIP10>3.0.CO;2-7/asset/10_ftp.pdf?v=1&t=h0e0ip2g&s=5cb0295ab7c8a3bd303ef0df6736f300becfb4e2-
heal.journalNameBiopolymersen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2000-
heal.publisherWileyen
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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