PAF effects on MCP-1 and IL-6 secretion in U-937 monocytes in comparison with OxLDL and IL-1 beta effects
Φόρτωση...
Ημερομηνία
Συγγραφείς
Verouti, S. N.
Fragopoulou, E.
Karantonis, H. C.
Dimitriou, A. A.
Tselepis, A. D.
Antonopoulou, S.
Nomikos, T.
Demopoulos, C. A.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier Ireland
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Atherosclerosis
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Objective: To study the effects of PAF, in comparison with OxLDL and IL-1 beta on MCP-1 and IL-6 secretion from U-937 monocytes and to investigate the mechanism of its action. Methods: U-937 cell line was cultured in the presence or absence of PAF or OxLDL or IL-1 beta. Secretion of IL-6 and MCP-1 was measured by ELISA method, mRNA levels of MCP-1 and PAFR was measured using real-time PCR. In order to investigate the mechanism of mediator's action signal transduction appropriate inhibitors was used and oxidant status of cells by measurement the total cellular thiols content and glutathione was determined. Results and conclusion: None of the tested mediators induced the secretion of IL-6. On the other hand PAF and OxLDL caused a short-term while IL-1 beta caused a long-term secretion and expression of MCP-1. Reduced total thiol levels and GSH/GSSG ratio indicate that the above mediators induce oxidative stress. The signal transduction of all mediators is mediated through G-proteins, protein kinases (PKC, serine-threonine kinase and tyrosine kinase) and NF-kappa B activation. In addition, PAF, OxLDL, IL-1 beta activates monocytes leading to increased PAF receptor mRNA levels. These results indicate that PAF and OxLDL, in a different pattern from that of IL-1 beta, regulate MCP-1 expression via pathways that involve changes in cell redox status. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
monocytes, paf, oxldl, oxidative stress, cytokines, inflammation, glutathione, platelet-activating-factor, low-density-lipoprotein, smooth-muscle-cells, protein-kinase-c, chemoattractant protein-1, kappa-b, endothelial-cells, u937 cells, oxidized phospholipids, oxidative modification
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000298813900024
http://ac.els-cdn.com/S0021915011007441/1-s2.0-S0021915011007441-main.pdf?_tid=41acd87b85c4c3a3d0ccf1596e8529df&acdnat=1333112381_803479283614e0834b4556c63a7020a6
http://ac.els-cdn.com/S0021915011007441/1-s2.0-S0021915011007441-main.pdf?_tid=41acd87b85c4c3a3d0ccf1596e8529df&acdnat=1333112381_803479283614e0834b4556c63a7020a6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας