PAF effects on MCP-1 and IL-6 secretion in U-937 monocytes in comparison with OxLDL and IL-1 beta effects

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dc.contributor.authorVerouti, S. N.en
dc.contributor.authorFragopoulou, E.en
dc.contributor.authorKarantonis, H. C.en
dc.contributor.authorDimitriou, A. A.en
dc.contributor.authorTselepis, A. D.en
dc.contributor.authorAntonopoulou, S.en
dc.contributor.authorNomikos, T.en
dc.contributor.authorDemopoulos, C. A.en
dc.date.accessioned2015-11-24T16:43:49Z
dc.date.available2015-11-24T16:43:49Z
dc.identifier.issn0021-9150-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/8741
dc.rightsDefault Licence-
dc.subjectmonocytesen
dc.subjectpafen
dc.subjectoxldlen
dc.subjectoxidative stressen
dc.subjectcytokinesen
dc.subjectinflammationen
dc.subjectglutathioneen
dc.subjectplatelet-activating-factoren
dc.subjectlow-density-lipoproteinen
dc.subjectsmooth-muscle-cellsen
dc.subjectprotein-kinase-cen
dc.subjectchemoattractant protein-1en
dc.subjectkappa-ben
dc.subjectendothelial-cellsen
dc.subjectu937 cellsen
dc.subjectoxidized phospholipidsen
dc.subjectoxidative modificationen
dc.titlePAF effects on MCP-1 and IL-6 secretion in U-937 monocytes in comparison with OxLDL and IL-1 beta effectsen
heal.abstractObjective: To study the effects of PAF, in comparison with OxLDL and IL-1 beta on MCP-1 and IL-6 secretion from U-937 monocytes and to investigate the mechanism of its action. Methods: U-937 cell line was cultured in the presence or absence of PAF or OxLDL or IL-1 beta. Secretion of IL-6 and MCP-1 was measured by ELISA method, mRNA levels of MCP-1 and PAFR was measured using real-time PCR. In order to investigate the mechanism of mediator's action signal transduction appropriate inhibitors was used and oxidant status of cells by measurement the total cellular thiols content and glutathione was determined. Results and conclusion: None of the tested mediators induced the secretion of IL-6. On the other hand PAF and OxLDL caused a short-term while IL-1 beta caused a long-term secretion and expression of MCP-1. Reduced total thiol levels and GSH/GSSG ratio indicate that the above mediators induce oxidative stress. The signal transduction of all mediators is mediated through G-proteins, protein kinases (PKC, serine-threonine kinase and tyrosine kinase) and NF-kappa B activation. In addition, PAF, OxLDL, IL-1 beta activates monocytes leading to increased PAF receptor mRNA levels. These results indicate that PAF and OxLDL, in a different pattern from that of IL-1 beta, regulate MCP-1 expression via pathways that involve changes in cell redox status. (C) 2011 Elsevier Ireland Ltd. All rights reserved.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1016/j.atherosclerosis.2011.07.123-
heal.identifier.secondary<Go to ISI>://000298813900024-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0021915011007441/1-s2.0-S0021915011007441-main.pdf?_tid=41acd87b85c4c3a3d0ccf1596e8529df&acdnat=1333112381_803479283614e0834b4556c63a7020a6-
heal.journalNameAtherosclerosisen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2011-
heal.publisherElsevier Irelanden
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείαςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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