A convenient and rapid method for the selective oxygen-17 enrichment of aspartyl peptides during solid-phase synthesis
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Theodorou-Kassioumis, V.
Biris, N.
Sakarellos, C.
Tsikaris, V.
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Elsevier
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peer reviewed
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Tetrahedron Lett
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In this work we describe, for the first time, a rapid and efficient method for O-17 selective labeling on the beta -carboxyl group of an aspartic acid residue already incorporated into a peptide sequence anchored on a solid-phase support. The beta -O-benzyl ester of the Asp residue of the Ac-RGD-benzydrylamine resin was successfully saponified using (NaOH)-O-17 in a methanol/ dichloromethane mixture. The O-17 selective enriched peptide was then released from the solid support by acidic cleavage. The O-17 NMR spectrum confirmed the O-17 labeling of the Asp beta -carboxylate. (C) 2001 Elsevier Science Ltd. All rights reserved.
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isotopic enrichment, oxygen-17, o-17 nmr, aspartic acid, rgd peptides, solid-phase o-17 peptide enrichment, o-17-nmr
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<Go to ISI>://000171556600042
http://ac.els-cdn.com/S004040390101632X/1-s2.0-S004040390101632X-main.pdf?_tid=74b29c30-3574-11e3-90ed-00000aab0f02&acdnat=1381826184_2f2f4259b5592d92ea527a7e8f4bea5f
http://ac.els-cdn.com/S004040390101632X/1-s2.0-S004040390101632X-main.pdf?_tid=74b29c30-3574-11e3-90ed-00000aab0f02&acdnat=1381826184_2f2f4259b5592d92ea527a7e8f4bea5f
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας