Microvascular repair with 1-mm polytetrafluoroethylene (PTFE) grafts: effect of recombinant tissue-type plasminogen activator (rt-PA) on the patency rate and healing process
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Lykoudis, E. G.
Papalois, A. E.
Gravvanis, A. I.
Frangia, K. B.
Stamatopoulos, C. N.
Ioannovich, J. D.
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peer-reviewed
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Microsurgery
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The present study assesses the effect of recombinant tissue-type plasminogen activator (rt-PA) on the patency rate and healing process of microvascular polytetrafluoroethylene (PTFE) grafts. Wistar rats were used, divided into four groups of 25 animals each. After dissection of the carotid artery a segment of the vessel, 1 cm long, was resected and replaced by equal length graft. Two different type fibril length (30- or 60-microm) grafts of the same wall thickness (0.18 mm) were used. Normal saline or 3 mg/kg of body weight of rt-PA was applied locally in each group of different fibril length grafts. Patency tests were performed at 15 min and 4 weeks after blood flow was reestablished. All grafts were harvested and examined histologically. The results showed that local application of rt-PA improves patency statistically significantly in both types of fibril length grafts. Patency in 60-microm fibril length grafts was statistically significantly higher than that of 30-microm fibril length grafts, whether rt-PA was used or not. The use of rt-PA had no influence on the healing process of either type of graft.
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Keywords
Animals, *Blood Vessel Prosthesis, Male, *Microsurgery, *Polytetrafluoroethylene, Rats, Rats, Wistar, Recombinant Proteins/*pharmacology, Tissue Plasminogen Activator/*pharmacology, Vascular Patency/*drug effects, Vascular Surgical Procedures, Wound Healing/*drug effects
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http://www.ncbi.nlm.nih.gov/pubmed/11119287
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en
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Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής