A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains
Φόρτωση...
Ημερομηνία
Συγγραφείς
Pliaka, V.
Dedepsidis, E.
Kyriakopoulou, Z.
Mpirli, K.
Tsakogiannis, D.
Pratti, A.
Levidiotou-Stefanou, S.
Markoulatos, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Mol Cell Probes
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free has switched from OPV (oral poliovirus vaccine) to IPV (inactivated poliovirus vaccine), importation of recombinant evolved derivatives of vaccinal strains would have serious implication for public health. To test the robustness of the proposed RT-PCR screening analysis, eleven recombinant vaccine-derived polioviruses that were characterized previously by sequencing by our group, in addition to three recently identified recombinant environmental isolates were assayed. Although the most definitive characterization of VDPVs is by genomic sequencing, in this study we describe a new, inexpensive and broadly applicable RT-PCR assay for the identification of the predominant recombination types S3/Sx in 2C and S2/Sx in 3D genomic regions respectively of VDPVs, that can be readily implemented in laboratories lacking sequencing facilities as a first approach for the early detection of vaccine-derived poliovirus (VDPVs).
Περιγραφή
Λέξεις-κλειδιά
DNA Primers/genetics, Genome, Viral/*genetics, Humans, Molecular Sequence Data, Poliomyelitis/immunology/virology, Poliovirus/*genetics/immunology/isolation & purification, Poliovirus Vaccine, Inactivated/immunology, Poliovirus Vaccine, Oral/immunology, RNA, Viral/*genetics, Recombination, Genetic, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction/*methods, Sequence Analysis, DNA, Sewage/virology, Virus Shedding
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/19948215
http://ac.els-cdn.com/S0890850809000802/1-s2.0-S0890850809000802-main.pdf?_tid=4839859286c42909be8dbde6b24d17db&acdnat=1332827088_740782191ae024981a946895222578a6
http://ac.els-cdn.com/S0890850809000802/1-s2.0-S0890850809000802-main.pdf?_tid=4839859286c42909be8dbde6b24d17db&acdnat=1332827088_740782191ae024981a946895222578a6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής