A new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strains

Απλή σελίδα τεκμηρίου
dc.contributor.authorPliaka, V.en
dc.contributor.authorDedepsidis, E.en
dc.contributor.authorKyriakopoulou, Z.en
dc.contributor.authorMpirli, K.en
dc.contributor.authorTsakogiannis, D.en
dc.contributor.authorPratti, A.en
dc.contributor.authorLevidiotou-Stefanou, S.en
dc.contributor.authorMarkoulatos, P.en
dc.date.accessioned2015-11-24T19:40:09Z
dc.date.available2015-11-24T19:40:09Z
dc.identifier.issn1096-1194-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/24306
dc.rightsDefault Licence-
dc.subjectDNA Primers/geneticsen
dc.subjectGenome, Viral/*geneticsen
dc.subjectHumansen
dc.subjectMolecular Sequence Dataen
dc.subjectPoliomyelitis/immunology/virologyen
dc.subjectPoliovirus/*genetics/immunology/isolation & purificationen
dc.subjectPoliovirus Vaccine, Inactivated/immunologyen
dc.subjectPoliovirus Vaccine, Oral/immunologyen
dc.subjectRNA, Viral/*geneticsen
dc.subjectRecombination, Geneticen
dc.subjectReproducibility of Resultsen
dc.subjectReverse Transcriptase Polymerase Chain Reaction/*methodsen
dc.subjectSequence Analysis, DNAen
dc.subjectSewage/virologyen
dc.subjectVirus Sheddingen
dc.titleA new RT-PCR assay for the identification of the predominant recombination types in 2C and 3D genomic regions of vaccine-derived poliovirus strainsen
heal.abstractIn the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free has switched from OPV (oral poliovirus vaccine) to IPV (inactivated poliovirus vaccine), importation of recombinant evolved derivatives of vaccinal strains would have serious implication for public health. To test the robustness of the proposed RT-PCR screening analysis, eleven recombinant vaccine-derived polioviruses that were characterized previously by sequencing by our group, in addition to three recently identified recombinant environmental isolates were assayed. Although the most definitive characterization of VDPVs is by genomic sequencing, in this study we describe a new, inexpensive and broadly applicable RT-PCR assay for the identification of the predominant recombination types S3/Sx in 2C and S2/Sx in 3D genomic regions respectively of VDPVs, that can be readily implemented in laboratories lacking sequencing facilities as a first approach for the early detection of vaccine-derived poliovirus (VDPVs).en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1016/j.mcp.2009.11.003-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/19948215-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0890850809000802/1-s2.0-S0890850809000802-main.pdf?_tid=4839859286c42909be8dbde6b24d17db&acdnat=1332827088_740782191ae024981a946895222578a6-
heal.journalNameMol Cell Probesen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2010-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ