Effects of adrenergic agents on rat peritoneal macrophages activated in vitro by acetylated low-density lipoprotein
Φόρτωση...
Ημερομηνία
Συγγραφείς
Kondomerkos, D. J.
Kalamidas, S. A.
Michalis, L. K.
Kanavaros, P.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Pathol Res Pract
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Macrophages interact with modified lipoproteins and alter their functional status. In this study, the effects of the adrenergic agents adrenaline, isoproterenol, and dobutamine on macrophages activated with acetylated low-density lipoprotein were tested. The aim of this investigation was to determine whether adrenergic agents influence biologically significant functions of these cells in an in vitro model of macrophage-lipoprotein acute interaction. Rat peritoneal macrophages were incubated with acetylated low-density lipoprotein for 16 h, with or without adrenergic agents. Hydrogen peroxide and nitric oxide production and acid phosphatase activities in the supernatant and cell lysate were assayed. Adrenaline and isoproterenol inhibited the production of hydrogen peroxide, stimulated the production of nitric oxide, and increased the extracellular activity of acid phosphatase in the lipoprotein-activated cells. Dobutamine increased the extracellular, but decreased the intracellular acid phosphatase activity. Adrenaline and dobutamine also produced changes in the cell size and nuclear morphology of the macrophages. Macrophages are activated in vitro by acetylated low-density lipoprotein, and their functions and morphology are modified under the influence of adrenergic agents. Certain effects could be attributed to changes in cyclic AMP levels.
Περιγραφή
Λέξεις-κλειδιά
Acid Phosphatase/metabolism, Adrenergic Agonists/*pharmacology, Adrenergic alpha-Agonists/pharmacology, Adrenergic beta-Agonists/pharmacology, Animals, Cell Shape/drug effects, Cells, Cultured, Dobutamine/pharmacology, Dose-Response Relationship, Drug, Epinephrine/pharmacology, Hydrogen Peroxide/metabolism, Isoproterenol/pharmacology, Lipopolysaccharides/pharmacology, Lipoproteins, LDL/*pharmacology, Macrophage Activation/*drug effects, Macrophages, Peritoneal/cytology/*drug effects/metabolism, Nitric Oxide/metabolism, Nitrites/metabolism, Rats
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/16047949
http://ac.els-cdn.com/S0344033805000634/1-s2.0-S0344033805000634-main.pdf?_tid=ddc130743fa0eee7f1f56309d769371b&acdnat=1333090614_4484140746f42aa306b25bd04e822c06
http://ac.els-cdn.com/S0344033805000634/1-s2.0-S0344033805000634-main.pdf?_tid=ddc130743fa0eee7f1f56309d769371b&acdnat=1333090614_4484140746f42aa306b25bd04e822c06
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής