Effect of Transosseous Application of Low-Intensity Ultrasound at the Tendon Graft-Bone Interface Healing: Gene Expression and Histological Analysis in Rabbits
Φόρτωση...
Ημερομηνία
Συγγραφείς
Papatheodorou, L. K.
Malizos, K. N.
Poultsides, L. A.
Hantes, M. E.
Grafanaki, K.
Giannouli, S.
Ioannou, M. G.
Koukoulis, G. K.
Protopappas, V. C.
Fotiadis, D. I.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Ultrasound in Medicine and Biology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in both knees of 52 New Zealand White rabbits was excised and replaced with the long digital extensor. A custom-made ultrasound transducer was implanted onto the medial tibial condyle, adjacent to the surface of the bone tunnel at both knees of the rabbits. The LiUS-treated right knees received 200-mu s bursts of 1 MHz sine waves at a pulse repetition rate of 1 kHz and with 30 MW/cm(2) spatial-average temporal-average intensity for 20 min daily (study group), while the left knee received no LiUS (control group). Thirty-six rabbits were used to perform semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis from both study and control groups for transforming growth factor-beta 1 (TGF-beta 1), biglycan and collagen I. RT-PCR products showed statistically significant upregulation of biglycan and collagen I gene expression in the study group, while TGF-beta 1 gene expression exhibited a bimodal profile. Histological examination performed in 16 rabbits from both groups supported the findings of the molecular analysis, indicating a faster healing rate and a more efficient ligamentization process after ultrasound treatment. These findings suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of genes significant for the tendon to bone healing process. (E-mail: malizos@med.uth.gr) (c) 2009 World Federation for Ultrasound in Medicine & Biology.
Περιγραφή
Λέξεις-κλειδιά
low-intensity ultrasound (lius), tendon graft-bone interface healing, tgf-beta 1, biglycan, collagen type i, anterior cruciate ligament (acl), anterior cruciate ligament, messenger-rna levels, pulsed ultrasound, growth-factors, patellar tendon, transforming growth-factor-beta-1, autogenous patellar, collagen-synthesis, human-fibroblasts, matrix synthesis
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000264977300007
http://ac.els-cdn.com/S0301562908003189/1-s2.0-S0301562908003189-main.pdf?_tid=e4d16a4d2c404021c5fe630029f85292&acdnat=1339758645_b7b6e8c23c877b203f70157d2e32e3c8
http://ac.els-cdn.com/S0301562908003189/1-s2.0-S0301562908003189-main.pdf?_tid=e4d16a4d2c404021c5fe630029f85292&acdnat=1339758645_b7b6e8c23c877b203f70157d2e32e3c8
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικών