Effect of Transosseous Application of Low-Intensity Ultrasound at the Tendon Graft-Bone Interface Healing: Gene Expression and Histological Analysis in Rabbits

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dc.contributor.authorPapatheodorou, L. K.en
dc.contributor.authorMalizos, K. N.en
dc.contributor.authorPoultsides, L. A.en
dc.contributor.authorHantes, M. E.en
dc.contributor.authorGrafanaki, K.en
dc.contributor.authorGiannouli, S.en
dc.contributor.authorIoannou, M. G.en
dc.contributor.authorKoukoulis, G. K.en
dc.contributor.authorProtopappas, V. C.en
dc.contributor.authorFotiadis, D. I.en
dc.contributor.authorStathopoulos, C.en
dc.date.accessioned2015-11-24T17:33:33Z
dc.date.available2015-11-24T17:33:33Z
dc.identifier.issn0301-5629-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/13873
dc.rightsDefault Licence-
dc.subjectlow-intensity ultrasound (lius)en
dc.subjecttendon graft-bone interface healingen
dc.subjecttgf-beta 1en
dc.subjectbiglycanen
dc.subjectcollagen type ien
dc.subjectanterior cruciate ligament (acl)en
dc.subjectanterior cruciate ligamenten
dc.subjectmessenger-rna levelsen
dc.subjectpulsed ultrasounden
dc.subjectgrowth-factorsen
dc.subjectpatellar tendonen
dc.subjecttransforming growth-factor-beta-1en
dc.subjectautogenous patellaren
dc.subjectcollagen-synthesisen
dc.subjecthuman-fibroblastsen
dc.subjectmatrix synthesisen
dc.titleEffect of Transosseous Application of Low-Intensity Ultrasound at the Tendon Graft-Bone Interface Healing: Gene Expression and Histological Analysis in Rabbitsen
heal.abstractThe present study investigates the effect of transosseous low-intensity pulsed ultrasound (LiUS) on the healing at tendon graft-bone interface, in molecular and histological level. The anterior cruciate ligament (ACL) in both knees of 52 New Zealand White rabbits was excised and replaced with the long digital extensor. A custom-made ultrasound transducer was implanted onto the medial tibial condyle, adjacent to the surface of the bone tunnel at both knees of the rabbits. The LiUS-treated right knees received 200-mu s bursts of 1 MHz sine waves at a pulse repetition rate of 1 kHz and with 30 MW/cm(2) spatial-average temporal-average intensity for 20 min daily (study group), while the left knee received no LiUS (control group). Thirty-six rabbits were used to perform semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis from both study and control groups for transforming growth factor-beta 1 (TGF-beta 1), biglycan and collagen I. RT-PCR products showed statistically significant upregulation of biglycan and collagen I gene expression in the study group, while TGF-beta 1 gene expression exhibited a bimodal profile. Histological examination performed in 16 rabbits from both groups supported the findings of the molecular analysis, indicating a faster healing rate and a more efficient ligamentization process after ultrasound treatment. These findings suggest that transosseous application of LiUS enhances the healing rate of the tendon graft-bone interface, possibly by affecting the expression levels of genes significant for the tendon to bone healing process. (E-mail: malizos@med.uth.gr) (c) 2009 World Federation for Ultrasound in Medicine & Biology.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primaryDOI 10.1016/j.ultrasmedbio.2008.07.003-
heal.identifier.secondary<Go to ISI>://000264977300007-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0301562908003189/1-s2.0-S0301562908003189-main.pdf?_tid=e4d16a4d2c404021c5fe630029f85292&acdnat=1339758645_b7b6e8c23c877b203f70157d2e32e3c8-
heal.journalNameUltrasound in Medicine and Biologyen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate2009-
heal.publisherElsevieren
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Επιστήμης Υλικώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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