The sequence and antiapoptotic functional domains of the human cytomegalovirus UL37 exon 1 immediate early protein are conserved in multiple primary strains
Φόρτωση...
Ημερομηνία
Συγγραφείς
Hayajneh, W. A.
Colberg-Poley, A. M.
Skaletskaya, A.
Bartle, L. M.
Lesperance, M. M.
Contopoulos-Ioannidis, D. G.
Kedersha, N. L.
Goldmacher, V. S.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Virology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The human cytomegalovirus UL37 exon 1 gene encodes the immediate early protein pUL37x1 that has antiapoptotic and regulatory activities. Deletion mutagenesis analysis of the open reading frame of UL37x1 identified two domains that are necessary and sufficient for its antiapoptotic activity. These domains are confined within the segments between amino acids 5 to 34, and 118 to 147, respectively. The first domain provides the targeting of the protein to mitochondria. Direct PCR sequencing of UL37 exon 1 amplified from 26 primary strains of human cytomegalovirus demonstrated that the promoter, polyadenylation signal, and the two segments of pUL37x1 required for its antiapoptotic function were invariant in all sequenced strains and identical to those in AD169 pUL37x1. In total, UL37 exon 1 varies between 0.0 and 1.6% at the nucleotide level from strain AD169. Only 11 amino acids were found to vary in one or more viral strains, and these variations occurred only in the domains of pUL37x1 dispensable for its antiapoptotic function. We infer from this remarkable conservation of pUL37x1 in primary strains that this protein and, probably, its antiapoptotic function are required for productive replication of human cytomegalovirus in humans.
Περιγραφή
Λέξεις-κλειδιά
Amino Acid Sequence, *Apoptosis/drug effects/physiology, Conserved Sequence, Cytomegalovirus/chemistry/*genetics/*physiology, Exons/*genetics, Gene Deletion, HeLa Cells, Humans, Immediate-Early Proteins/*chemistry/*genetics/*physiology, Molecular Sequence Data, Polymerase Chain Reaction/methods, Sequence Deletion, Structure-Activity Relationship, *Viral Proteins
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/11145905
http://ac.els-cdn.com/S0042682200907268/1-s2.0-S0042682200907268-main.pdf?_tid=bac0c12ae004de7768de9532b7b94951&acdnat=1332914335_b468d9423cc643b5c450b48745e8e1b6
http://ac.els-cdn.com/S0042682200907268/1-s2.0-S0042682200907268-main.pdf?_tid=bac0c12ae004de7768de9532b7b94951&acdnat=1332914335_b468d9423cc643b5c450b48745e8e1b6
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής