The sequence and antiapoptotic functional domains of the human cytomegalovirus UL37 exon 1 immediate early protein are conserved in multiple primary strains

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dc.contributor.authorHayajneh, W. A.en
dc.contributor.authorColberg-Poley, A. M.en
dc.contributor.authorSkaletskaya, A.en
dc.contributor.authorBartle, L. M.en
dc.contributor.authorLesperance, M. M.en
dc.contributor.authorContopoulos-Ioannidis, D. G.en
dc.contributor.authorKedersha, N. L.en
dc.contributor.authorGoldmacher, V. S.en
dc.date.accessioned2015-11-24T19:24:55Z
dc.date.available2015-11-24T19:24:55Z
dc.identifier.issn0042-6822-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/22543
dc.rightsDefault Licence-
dc.subjectAmino Acid Sequenceen
dc.subject*Apoptosis/drug effects/physiologyen
dc.subjectConserved Sequenceen
dc.subjectCytomegalovirus/chemistry/*genetics/*physiologyen
dc.subjectExons/*geneticsen
dc.subjectGene Deletionen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectImmediate-Early Proteins/*chemistry/*genetics/*physiologyen
dc.subjectMolecular Sequence Dataen
dc.subjectPolymerase Chain Reaction/methodsen
dc.subjectSequence Deletionen
dc.subjectStructure-Activity Relationshipen
dc.subject*Viral Proteinsen
dc.titleThe sequence and antiapoptotic functional domains of the human cytomegalovirus UL37 exon 1 immediate early protein are conserved in multiple primary strainsen
heal.abstractThe human cytomegalovirus UL37 exon 1 gene encodes the immediate early protein pUL37x1 that has antiapoptotic and regulatory activities. Deletion mutagenesis analysis of the open reading frame of UL37x1 identified two domains that are necessary and sufficient for its antiapoptotic activity. These domains are confined within the segments between amino acids 5 to 34, and 118 to 147, respectively. The first domain provides the targeting of the protein to mitochondria. Direct PCR sequencing of UL37 exon 1 amplified from 26 primary strains of human cytomegalovirus demonstrated that the promoter, polyadenylation signal, and the two segments of pUL37x1 required for its antiapoptotic function were invariant in all sequenced strains and identical to those in AD169 pUL37x1. In total, UL37 exon 1 varies between 0.0 and 1.6% at the nucleotide level from strain AD169. Only 11 amino acids were found to vary in one or more viral strains, and these variations occurred only in the domains of pUL37x1 dispensable for its antiapoptotic function. We infer from this remarkable conservation of pUL37x1 in primary strains that this protein and, probably, its antiapoptotic function are required for productive replication of human cytomegalovirus in humans.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.primary10.1006/viro.2000.0726-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/11145905-
heal.identifier.secondaryhttp://ac.els-cdn.com/S0042682200907268/1-s2.0-S0042682200907268-main.pdf?_tid=bac0c12ae004de7768de9532b7b94951&acdnat=1332914335_b468d9423cc643b5c450b48745e8e1b6-
heal.journalNameVirologyen
heal.journalTypepeer-reviewed-
heal.languageen-
heal.publicationDate2001-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

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