Glycine maintains mitochondrial activity and bile composition following warm liver ischemia-reperfusion injury
Φόρτωση...
Ημερομηνία
Συγγραφείς
Sheth, H.
Hafez, T.
Glantzounis, G. K.
Seifalian, A. M.
Fuller, B.
Davidson, B. R.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Gastroenterol Hepatol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND AND AIM: Experimental studies have shown protective effect by the non-essential amino acid glycine to liver ischemia-reperfusion (I/R) injury but the mechanism of action is unknown. METHODS: A rabbit model of hepatic lobar I/R was used. Three groups of animals (n=6) were studied: Sham group (laparotomy alone), ischemia reperfusion (I/R) group (1 h of liver lobar ischemia and 6 h of reperfusion), and a glycine I/R group (intravenous glycine 5 mg/kg prior to the I/R protocol). Systemic and hepatic hemodynamics, degree of liver injury (bile flow, transaminases), hepatic microcirculation, mitochondrial activity (redox state of cytochrome oxidase), bile composition and cytokines (tumor necrosis factor-alpha and interleukin-8) were measured during the experiment. RESULTS: Glycine administration increased portal blood flow, bile production, hepatic microcirculation and maintained cytochrome oxidase activity as compared with the I/R group during reperfusion. Glycine also reduced bile lactate surge and stimulated acetoacetate release in bile during reperfusion versus the I/R group. Cytokine levels (tumor necrosis factor-alpha, interleukin-8) and hepatocellular injury (aspartate aminotransferase and alanine aminotransferase) were significantly reduced by glycine administration. CONCLUSION: Intravenous glycine administration reduces liver warm I/R injury by reducing the systemic inflammatory response, and maintaining cellular energy production.
Περιγραφή
Λέξεις-κλειδιά
Alanine Transaminase/blood, Animals, Aspartate Aminotransferases/blood, Bile/*metabolism, Disease Models, Animal, Electron Transport Complex IV/metabolism, Energy Metabolism/*drug effects, Glycine/administration & dosage/*pharmacology, Hemodynamics/drug effects, Infusions, Intravenous, Interleukin-8/blood, Liver/*blood supply/*drug effects/metabolism, Liver Circulation/drug effects, Microcirculation/drug effects, Mitochondria, Liver/*drug effects/metabolism, Oxidation-Reduction, Rabbits, Reperfusion Injury/metabolism/physiopathology/*prevention & control, Time Factors, Tumor Necrosis Factor-alpha/blood, Warm Ischemia/*adverse effects
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/21175814
http://onlinelibrary.wiley.com/store/10.1111/j.1440-1746.2010.06323.x/asset/j.1440-1746.2010.06323.x.pdf?v=1&t=h0nqjx9j&s=dcfbfdac8d4a4d9771b28742e816dac668c68a0c
http://onlinelibrary.wiley.com/store/10.1111/j.1440-1746.2010.06323.x/asset/j.1440-1746.2010.06323.x.pdf?v=1&t=h0nqjx9j&s=dcfbfdac8d4a4d9771b28742e816dac668c68a0c
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής