Glycine maintains mitochondrial activity and bile composition following warm liver ischemia-reperfusion injury
Φόρτωση...
Ημερομηνία
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
J Gastroenterol Hepatol
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
BACKGROUND AND AIM: Experimental studies have shown protective effect by the non-essential amino acid glycine to liver ischemia-reperfusion (I/R) injury but the mechanism of action is unknown. METHODS: A rabbit model of hepatic lobar I/R was used. Three groups of animals (n=6) were studied: Sham group (laparotomy alone), ischemia reperfusion (I/R) group (1 h of liver lobar ischemia and 6 h of reperfusion), and a glycine I/R group (intravenous glycine 5 mg/kg prior to the I/R protocol). Systemic and hepatic hemodynamics, degree of liver injury (bile flow, transaminases), hepatic microcirculation, mitochondrial activity (redox state of cytochrome oxidase), bile composition and cytokines (tumor necrosis factor-alpha and interleukin-8) were measured during the experiment. RESULTS: Glycine administration increased portal blood flow, bile production, hepatic microcirculation and maintained cytochrome oxidase activity as compared with the I/R group during reperfusion. Glycine also reduced bile lactate surge and stimulated acetoacetate release in bile during reperfusion versus the I/R group. Cytokine levels (tumor necrosis factor-alpha, interleukin-8) and hepatocellular injury (aspartate aminotransferase and alanine aminotransferase) were significantly reduced by glycine administration. CONCLUSION: Intravenous glycine administration reduces liver warm I/R injury by reducing the systemic inflammatory response, and maintaining cellular energy production.
Περιγραφή
Λέξεις-κλειδιά
Alanine Transaminase/blood, Animals, Aspartate Aminotransferases/blood, Bile/*metabolism, Disease Models, Animal, Electron Transport Complex IV/metabolism, Energy Metabolism/*drug effects, Glycine/administration & dosage/*pharmacology, Hemodynamics/drug effects, Infusions, Intravenous, Interleukin-8/blood, Liver/*blood supply/*drug effects/metabolism, Liver Circulation/drug effects, Microcirculation/drug effects, Mitochondria, Liver/*drug effects/metabolism, Oxidation-Reduction, Rabbits, Reperfusion Injury/metabolism/physiopathology/*prevention & control, Time Factors, Tumor Necrosis Factor-alpha/blood, Warm Ischemia/*adverse effects
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/21175814
http://onlinelibrary.wiley.com/store/10.1111/j.1440-1746.2010.06323.x/asset/j.1440-1746.2010.06323.x.pdf?v=1&t=h0nqjx9j&s=dcfbfdac8d4a4d9771b28742e816dac668c68a0c
http://onlinelibrary.wiley.com/store/10.1111/j.1440-1746.2010.06323.x/asset/j.1440-1746.2010.06323.x.pdf?v=1&t=h0nqjx9j&s=dcfbfdac8d4a4d9771b28742e816dac668c68a0c
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής