Detoxification of 2,4-dichlorophenol by the marine microalga Tetraselmis marina
Φόρτωση...
Ημερομηνία
Συγγραφείς
Petroutsos, D.
Katapodis, P.
Samiotaki, M.
Panayotou, G.
Kekos, D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Phytochemistry
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
Xenobiotic chlorinated phenols have been found in fresh and marine waters and are toxic to many aquatic organisms. Metabolism of 2,4-dichlorophenol (2,4-DCP) in the marine microalga Tetraselmis marina was studied. The microalga removed more than 1mM of 2,4-DCP in a 2l photobioreactor over a 6 day period. Two metabolites, more polar than 2,4-DCP, were detected in the growth medium by reverse phase HPLC and their concentrations increased at the expense of 2,4-DCP. The metabolites were isolated by a C8 HPLC column and identified as 2,4-dichlorophenyl-beta-d-glucopyranoside (DCPG) and 2,4-dichlorophenyl-beta-d-(6-O-malonyl)-glucopyranoside (DCPGM) by electrospray ionization-mass spectrometric analysis in a negative ion mode. The molecular structures of 2,4-DCPG and 2,4-CPGM were further confirmed by enzymatic and alkaline hydrolyses. Thus, it was concluded that the major pathway of 2,4-DCP metabolism in T. marina involves an initial conjugation of 2,4-DCP to glucose to form 2,4-dichlorophenyl-beta-d-glucopyranoside, followed by acylation of the glucoconjugate to form 2,4-dichlorophenyl-beta-d-(6-O-malonyl)-glucopyranoside. The microalga ability to detoxify dichlorophenol congeners other than 2,4-DCP was also investigated. This work provides the first evidence that microalgae can use a combined glucosyl and malonyl transfer to detoxify xenobiotics such as dichlorophenols.
Περιγραφή
Λέξεις-κλειδιά
Bioreactors, Chlorophenols/chemistry/*metabolism/toxicity, Chlorophyta/drug effects/growth & development/*metabolism, Chromatography, High Pressure Liquid/methods, Molecular Structure, Stereoisomerism, Time Factors, Toxicity Tests, Water Pollutants, Che
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/17936864
http://ac.els-cdn.com/S0031942207005420/1-s2.0-S0031942207005420-main.pdf?_tid=0ccddb0a-c389-11e2-b92e-00000aab0f6b&acdnat=1369300596_b399b47e3a30872a97febb17cfa25afb
http://ac.els-cdn.com/S0031942207005420/1-s2.0-S0031942207005420-main.pdf?_tid=0ccddb0a-c389-11e2-b92e-00000aab0f6b&acdnat=1369300596_b399b47e3a30872a97febb17cfa25afb
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών