Detoxification of 2,4-dichlorophenol by the marine microalga Tetraselmis marina
| dc.contributor.author | Petroutsos, D. | en |
| dc.contributor.author | Katapodis, P. | en |
| dc.contributor.author | Samiotaki, M. | en |
| dc.contributor.author | Panayotou, G. | en |
| dc.contributor.author | Kekos, D. | en |
| dc.date.accessioned | 2015-11-24T16:34:06Z | |
| dc.date.available | 2015-11-24T16:34:06Z | |
| dc.identifier.issn | 0031-9422 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/7757 | |
| dc.rights | Default Licence | - |
| dc.subject | Bioreactors | en |
| dc.subject | Chlorophenols/chemistry/*metabolism/toxicity | en |
| dc.subject | Chlorophyta/drug effects/growth & development/*metabolism | en |
| dc.subject | Chromatography, High Pressure Liquid/methods | en |
| dc.subject | Molecular Structure | en |
| dc.subject | Stereoisomerism | en |
| dc.subject | Time Factors | en |
| dc.subject | Toxicity Tests | en |
| dc.subject | Water Pollutants, Che | en |
| dc.title | Detoxification of 2,4-dichlorophenol by the marine microalga Tetraselmis marina | en |
| heal.abstract | Xenobiotic chlorinated phenols have been found in fresh and marine waters and are toxic to many aquatic organisms. Metabolism of 2,4-dichlorophenol (2,4-DCP) in the marine microalga Tetraselmis marina was studied. The microalga removed more than 1mM of 2,4-DCP in a 2l photobioreactor over a 6 day period. Two metabolites, more polar than 2,4-DCP, were detected in the growth medium by reverse phase HPLC and their concentrations increased at the expense of 2,4-DCP. The metabolites were isolated by a C8 HPLC column and identified as 2,4-dichlorophenyl-beta-d-glucopyranoside (DCPG) and 2,4-dichlorophenyl-beta-d-(6-O-malonyl)-glucopyranoside (DCPGM) by electrospray ionization-mass spectrometric analysis in a negative ion mode. The molecular structures of 2,4-DCPG and 2,4-CPGM were further confirmed by enzymatic and alkaline hydrolyses. Thus, it was concluded that the major pathway of 2,4-DCP metabolism in T. marina involves an initial conjugation of 2,4-DCP to glucose to form 2,4-dichlorophenyl-beta-d-glucopyranoside, followed by acylation of the glucoconjugate to form 2,4-dichlorophenyl-beta-d-(6-O-malonyl)-glucopyranoside. The microalga ability to detoxify dichlorophenol congeners other than 2,4-DCP was also investigated. This work provides the first evidence that microalgae can use a combined glucosyl and malonyl transfer to detoxify xenobiotics such as dichlorophenols. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1016/j.phytochem.2007.09.002 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/17936864 | - |
| heal.identifier.secondary | http://ac.els-cdn.com/S0031942207005420/1-s2.0-S0031942207005420-main.pdf?_tid=0ccddb0a-c389-11e2-b92e-00000aab0f6b&acdnat=1369300596_b399b47e3a30872a97febb17cfa25afb | - |
| heal.journalName | Phytochemistry | en |
| heal.journalType | peer reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 2008 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιών | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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