Increased manganese superoxide dismutase activity, protein, and mRNA levels and concurrent induction of tumor necrosis factor alpha in radiation-initiated Syrian hamster cells
Φόρτωση...
Ημερομηνία
Συγγραφείς
Otero, G.
Avila, M. A.
Emfietzoglou, D.
Clerch, L. B.
Massaro, D.
Notario, V.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Mol Carcinog
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The levels of the antioxidant enzyme manganese superoxide dismutase (Mn-SOD) are frequently decreased in tumor cells and increased in normal cells upon treatment with ionizing radiation. We studied Mn-SOD at different stages during the neoplastic conversion of radiation-initiated Syrian hamster embryo HDR-3 cells. Mn-SOD activity and the concentration of Mn-SOD protein and mRNA increased gradually during the malignant transformation of HDR-3 cells after radiation exposure; fully neoplastic cells showed greater Mn-SOD levels than preneoplastic and normal 84-3 cells. Inhibitors of superoxide (SO) anion production (thenoyltrifluoroacetone and rotenone) decreased the concentration of Mn-SOD mRNA, raising the possibility that the generation of SO radicals participated in the upregulation of Mn-SOD in cells transformed by exposure to radiation. We observed an increase in the concentration of tumor necrosis factor alpha (TNF alpha) in HDR-3 cells relative to mock-irradiated cells. Together with the observation that TNF alpha stimulates the production of SO by mitochondria and increases the level of Mn-SOD mRNA in other experimental systems, our results suggest that as normal 84-3 cells undergo malignant transformation induced by ionizing radiation they produce TNF alpha, to which the cells respond by increasing the concentration of Mn-SOD mRNA and protein and the activity of the enzyme.
Περιγραφή
Λέξεις-κλειδιά
Animals, Cell Transformation, Neoplastic/*radiation effects, Cells, Cultured, Cricetinae, Embryo, Mammalian, Fibroblasts/*metabolism/*radiation effects, Mesocricetus, Mitochondria/metabolism/radiation effects, RNA, Messenger/*metabolism, Superoxide Dismutase/*metabolism, Tumor Necrosis Factor-alpha/*biosynthesis, Up-Regulation/radiation effects
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/8989910
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1098-2744(199612)17:4<175::AID-MC1>3.0.CO;2-D/asset/1_ftp.pdf?v=1&t=h0ko2qp2&s=551c20377220b8d80757812671b41f24431f44e0
http://onlinelibrary.wiley.com/store/10.1002/(SICI)1098-2744(199612)17:4<175::AID-MC1>3.0.CO;2-D/asset/1_ftp.pdf?v=1&t=h0ko2qp2&s=551c20377220b8d80757812671b41f24431f44e0
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής