Increased manganese superoxide dismutase activity, protein, and mRNA levels and concurrent induction of tumor necrosis factor alpha in radiation-initiated Syrian hamster cells
| dc.contributor.author | Otero, G. | en |
| dc.contributor.author | Avila, M. A. | en |
| dc.contributor.author | Emfietzoglou, D. | en |
| dc.contributor.author | Clerch, L. B. | en |
| dc.contributor.author | Massaro, D. | en |
| dc.contributor.author | Notario, V. | en |
| dc.date.accessioned | 2015-11-24T19:10:45Z | |
| dc.date.available | 2015-11-24T19:10:45Z | |
| dc.identifier.issn | 0899-1987 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/20866 | |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Cell Transformation, Neoplastic/*radiation effects | en |
| dc.subject | Cells, Cultured | en |
| dc.subject | Cricetinae | en |
| dc.subject | Embryo, Mammalian | en |
| dc.subject | Fibroblasts/*metabolism/*radiation effects | en |
| dc.subject | Mesocricetus | en |
| dc.subject | Mitochondria/metabolism/radiation effects | en |
| dc.subject | RNA, Messenger/*metabolism | en |
| dc.subject | Superoxide Dismutase/*metabolism | en |
| dc.subject | Tumor Necrosis Factor-alpha/*biosynthesis | en |
| dc.subject | Up-Regulation/radiation effects | en |
| dc.title | Increased manganese superoxide dismutase activity, protein, and mRNA levels and concurrent induction of tumor necrosis factor alpha in radiation-initiated Syrian hamster cells | en |
| heal.abstract | The levels of the antioxidant enzyme manganese superoxide dismutase (Mn-SOD) are frequently decreased in tumor cells and increased in normal cells upon treatment with ionizing radiation. We studied Mn-SOD at different stages during the neoplastic conversion of radiation-initiated Syrian hamster embryo HDR-3 cells. Mn-SOD activity and the concentration of Mn-SOD protein and mRNA increased gradually during the malignant transformation of HDR-3 cells after radiation exposure; fully neoplastic cells showed greater Mn-SOD levels than preneoplastic and normal 84-3 cells. Inhibitors of superoxide (SO) anion production (thenoyltrifluoroacetone and rotenone) decreased the concentration of Mn-SOD mRNA, raising the possibility that the generation of SO radicals participated in the upregulation of Mn-SOD in cells transformed by exposure to radiation. We observed an increase in the concentration of tumor necrosis factor alpha (TNF alpha) in HDR-3 cells relative to mock-irradiated cells. Together with the observation that TNF alpha stimulates the production of SO by mitochondria and increases the level of Mn-SOD mRNA in other experimental systems, our results suggest that as normal 84-3 cells undergo malignant transformation induced by ionizing radiation they produce TNF alpha, to which the cells respond by increasing the concentration of Mn-SOD mRNA and protein and the activity of the enzyme. | en |
| heal.access | campus | - |
| heal.fullTextAvailability | TRUE | - |
| heal.identifier.primary | 10.1002/(SICI)1098-2744(199612)17:4<175::AID-MC1>3.0.CO;2-D | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/8989910 | - |
| heal.identifier.secondary | http://onlinelibrary.wiley.com/store/10.1002/(SICI)1098-2744(199612)17:4<175::AID-MC1>3.0.CO;2-D/asset/1_ftp.pdf?v=1&t=h0ko2qp2&s=551c20377220b8d80757812671b41f24431f44e0 | - |
| heal.journalName | Mol Carcinog | en |
| heal.journalType | peer-reviewed | - |
| heal.language | en | - |
| heal.publicationDate | 1996 | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.type | journalArticle | - |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.type.en | Journal article | en |
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