Immunohistochemical distribution of renal prostaglandin endoperoxide synthase and prostacyclin synthase: diminished endoperoxide synthase in the hepatorenal syndrome
Φόρτωση...
Ημερομηνία
Συγγραφείς
Govindarajan, S.
Nast, C. C.
Smith, W. L.
Koyle, M. A.
Daskalopoulos, G.
Zipser, R. D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer-reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Hepatology
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
To evaluate possible causes of the diminished prostaglandin production in advanced hepatorenal syndrome, prostaglandin endoperoxide synthase and prostacyclin synthase were localized and semiquantitated by immunofluorescence in postmortem, biopsy and nephrectomy renal tissues. In normal kidneys, antiprostacyclin synthase serum caused intense staining in peritubular capillaries, in the adjacent renal interstitial cells and in glomerular mesangial regions. Antiprostaglandin endoperoxide synthase serum caused staining of collecting duct epithelial cells, cells of the thin ascending limb and possibly glomerular mesangial cells. Prostacyclin synthase-positive staining was graded 5+ (scale of 0+ to 5+) in all kidney samples. Medullary collecting tubule prostaglandin endoperoxide synthase-positive staining was graded 4+ or 5+ in kidney samples from patients with acute tubular necrosis or acute tubulointerstitial nephritis and from patients with liver failure without the hepatorenal syndrome. However, prostaglandin endoperoxide synthase-positive staining was markedly diminished or absent (average 1+) in patients with the hepatorenal syndrome. These data suggest that loss of the medullary prostaglandin endoperoxide synthase is the cause of diminished urinary prostaglandin E2 excretion in the hepatorenal syndrome.
Περιγραφή
Λέξεις-κλειδιά
Adult, Aged, *Cytochrome P-450 Enzyme System, Dinoprostone, Epoprostenol/analysis/*biosynthesis, Fluorescent Antibody Technique, Hepatorenal Syndrome/*enzymology/urine, Humans, *Intramolecular Oxidoreductases, Kidney/analysis/*enzymology, Kidney Diseases/*enzymology, Kidney Failure, Chronic/metabolism, Middle Aged, Prostaglandin-Endoperoxide Synthases/*analysis, Prostaglandins E/urine
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
http://www.ncbi.nlm.nih.gov/pubmed/3111964
http://onlinelibrary.wiley.com/store/10.1002/hep.1840070407/asset/1840070407_ftp.pdf?v=1&t=h0p330nw&s=942f5c42cf0f08c459e32550c5d40d14118a2833
http://onlinelibrary.wiley.com/store/10.1002/hep.1840070407/asset/1840070407_ftp.pdf?v=1&t=h0p330nw&s=942f5c42cf0f08c459e32550c5d40d14118a2833
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής