Gas chromatographic determination of amino acids via one-step phase-transfer catalytic pentafluorobenzylation-preconcentration
Φόρτωση...
Ημερομηνία
Συγγραφείς
Fiamegos, Y. C.
Stalikas, C. D.
Τίτλος Εφημερίδας
Περιοδικό ISSN
Τίτλος τόμου
Εκδότης
Elsevier
Περίληψη
Τύπος
Είδος δημοσίευσης σε συνέδριο
Είδος περιοδικού
peer reviewed
Είδος εκπαιδευτικού υλικού
Όνομα συνεδρίου
Όνομα περιοδικού
Journal of Chromatography A
Όνομα βιβλίου
Σειρά βιβλίου
Έκδοση βιβλίου
Συμπληρωματικός/δευτερεύων τίτλος
Περιγραφή
The gas chromatographic determination of amino acids via their simultaneous extraction, preconcentration and pentafluorobenzylation is reported. Using phase-transfer catalysis (PTC), the amino acids under study were transformed to their pentafluorobenzyl adducts. The method was tested for different catalysts and tetrabutylammonium bromide provided favorable features in comparison to the other PTCs. The derivatization procedure was optimized and the best reaction conditions are given. With the exception of arginine, 19 amino acids were converted to volatile derivatives and analyzed with GC/MS and GC/FID at low concentration levels with acceptable sensitivity and good reproducibility. The LODs were found to range from 0.7 to 2.3 mu M for the GC/MS analyses and from 1.7 to 6.9 mu M for GC/FID analyses. The method practicability and applicability were confirmed by the analysis of urine, fruit juice and wheat flour for the determination of the amino acids under study. Protein-bound amino acids were analyzed after an alkaline hydrolysis step with 5 M NaOH applying this method to wheat flour with an overall procedure duration less than 12 h. The optimized protocol was applied to these samples without any pretreatment and their amino acid concentrations were calculated from the appropriate calibration plots. (c) 2006 Elsevier B.V. All rights reserved.
Περιγραφή
Λέξεις-κλειδιά
phase-transfer catalysis, amino acids, alkaline hydrolysis, gas chromatography, pentafluorobenzyl bromide, ionization mass-spectrometry, performance liquid-chromatography, chemical-ionization, capillary-electrophoresis, selective detection, quaternary ammonium, flow-injection, derivatization, derivatives, samples
Θεματική κατηγορία
Παραπομπή
Σύνδεσμος
<Go to ISI>://000236439000009
http://ac.els-cdn.com/S0021967306002068/1-s2.0-S0021967306002068-main.pdf?_tid=0db7af539555d361fc7a9efeea17daff&acdnat=1333040758_52d6b858b63b9c563f265f3aff27d348
http://ac.els-cdn.com/S0021967306002068/1-s2.0-S0021967306002068-main.pdf?_tid=0db7af539555d361fc7a9efeea17daff&acdnat=1333040758_52d6b858b63b9c563f265f3aff27d348
Γλώσσα
en
Εκδίδον τμήμα/τομέας
Όνομα επιβλέποντος
Εξεταστική επιτροπή
Γενική Περιγραφή / Σχόλια
Ίδρυμα και Σχολή/Τμήμα του υποβάλλοντος
Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας