Initiation of transcription at the human terminal deoxynucleotidyl transferase gene promoter: a novel role for the TATA binding protein

Απλή σελίδα τεκμηρίου
dc.contributor.authorSorscher, D. H.en
dc.contributor.authorYang, B.en
dc.contributor.authorBhaumik, D.en
dc.contributor.authorTrangas, T.en
dc.contributor.authorPhilips, A. V.en
dc.contributor.authorChancellor, K. E.en
dc.contributor.authorColeman, M. S.en
dc.date.accessioned2015-11-24T16:35:08Z
dc.date.available2015-11-24T16:35:08Z
dc.identifier.issn0006-2960-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/7905
dc.rightsDefault Licence-
dc.subjectBase Sequenceen
dc.subjectCell Lineen
dc.subjectDNA Nucleotidylexotransferase/*geneticsen
dc.subjectDNA Primersen
dc.subjectDNA-Binding Proteins/*metabolismen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectMolecular Sequence Dataen
dc.subjectMutagenesisen
dc.subjectMutationen
dc.subject*Promoter Regions, Geneticen
dc.subjectSequence Deletionen
dc.subject*TATA Boxen
dc.subjectTATA-Box Binden
dc.titleInitiation of transcription at the human terminal deoxynucleotidyl transferase gene promoter: a novel role for the TATA binding proteinen
heal.abstractControl of initiation of transcription of the human terminal deoxynucleotidyl transferase (TdT) gene was investigated by using an in vitro transcription assay. The precise contribution of discrete basal promoter elements to transcription initiation was determined by testing deletion and substitution mutations. The primary element, contained within the region spanning -34 to -14 bp relative to the transcription start site, accounted for 80% of basal promoter activity. TdT promoter activity required the sequence ACCCT at -24 to -20 bp since a dramatic decrease in transcription initiation was observed after mutation of this sequence, whereas mutation of the adjacent sequence from -32 to -25 bp did not alter promoter activity. The secondary element contained sequences surrounding the transcription start site and had 20% of promoter activity. Deletion of both elements completely abolished transcription initiation. Initiator characteristics of the secondary element were revealed by using the in vitro assay: promoter sequences at the transcription start site were sufficient to direct accurate initiation at a single site. Mutation of the sequence GGGTG spanning the transcription start site resulted in loss of transcription initiation. Both the primary and secondary elements were nonhomologous to corresponding regions from the mouse TdT gene promoter. While the human basal promoter functioned in the absence of TATA consensus sequences or GC-rich SP1 binding sites, it was dependent on active TFIID. In contrast to other TATA-less promoters, purified TATA binding protein substituted for the TFIID complex and restored promoter activity to TFIID-inactivated nuclear extracts.en
heal.accesscampus-
heal.fullTextAvailabilityTRUE-
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/8086419-
heal.journalNameBiochemistryen
heal.journalTypepeer reviewed-
heal.languageen-
heal.publicationDate1994-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών και Τεχνολογιών. Τμήμα Βιολογικών Εφαρμογών και Τεχνολογιώνel
heal.typejournalArticle-
heal.type.elΆρθρο Περιοδικούel
heal.type.enJournal articleen

Αρχεία

Φάκελος/Πακέτο αδειών

Προβολή: 1 - 1 of 1
Μικρογραφία εικόνας
Ονομα:
license.txt
Μέγεθος:
1.74 KB
Μορφότυπο:
Item-specific license agreed upon to submission
Περιγραφή:
Κατεβάστε

Συλλογές

Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)